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mtfp1 fluorescent protein  (Allele Biotechnology)

 
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    Structured Review

    Allele Biotechnology mtfp1 fluorescent protein
    Commonly Used Fluorescent Proteins
    Mtfp1 Fluorescent Protein, supplied by Allele Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtfp1+protein/mtfp1+fluorescent+protein/pmc07974382-109-3-0
    Average 90 stars, based on 1 article reviews
    mtfp1 fluorescent protein - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Fluorescent Proteins for Flow Cytometry"

    Article Title: Fluorescent Proteins for Flow Cytometry

    Journal: Current protocols in cytometry

    doi: 10.1002/cpcy.17

    Commonly Used Fluorescent Proteins
    Figure Legend Snippet: Commonly Used Fluorescent Proteins

    Techniques Used:

    Non-Profit and Commercial Sources of Fluorescent Proteins and Expression Vectors
    Figure Legend Snippet: Non-Profit and Commercial Sources of Fluorescent Proteins and Expression Vectors

    Techniques Used: Expressing, Plasmid Preparation, Recombinant, Luciferase, Selection, Marker

    Related Articles

    Variant Assay:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Irradiation:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Fluorescence:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Expressing:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Mutagenesis:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Incubation:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Comparison:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Clone Assay:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Plasmid Preparation:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Construct:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.

    Software:

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1
    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Article Title: Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores
    Article Snippet: The TN-L15 sensor, cloned into the mammalian expression vector pcDNA3, was a generous gift from O. Griesbeck . mTFP1 was obtained from Allele Biotechnology (pmTFP1-N vector). mTFP-TnC-Cit was constructed by substituting a single fluorophore from the original TN-L15 sensor.



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    Mtfp1 Fluorescent Protein, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtfp1+protein/mtfp1+fluorescent+protein/pmc03810607-229-7-19
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    mtfp1 fluorescent protein - by Bioz Stars, 2026-09
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    Commonly Used Fluorescent Proteins

    Journal: Current protocols in cytometry

    Article Title: Fluorescent Proteins for Flow Cytometry

    doi: 10.1002/cpcy.17

    Figure Lengend Snippet: Commonly Used Fluorescent Proteins

    Article Snippet: Allele Biotechnology , mTFP1, mWasabi, mMaple, mClavGR2, pcFPs, LanYFP, LanRFP, and mNeonGreen , http://www.allelebiotech.com/fluorescent-proteins.

    Techniques:

    Non-Profit and Commercial Sources of Fluorescent Proteins and Expression Vectors

    Journal: Current protocols in cytometry

    Article Title: Fluorescent Proteins for Flow Cytometry

    doi: 10.1002/cpcy.17

    Figure Lengend Snippet: Non-Profit and Commercial Sources of Fluorescent Proteins and Expression Vectors

    Article Snippet: Allele Biotechnology , mTFP1, mWasabi, mMaple, mClavGR2, pcFPs, LanYFP, LanRFP, and mNeonGreen , http://www.allelebiotech.com/fluorescent-proteins.

    Techniques: Expressing, Plasmid Preparation, Recombinant, Luciferase, Selection, Marker

    FRET-FLIM analysis of mutant SOD1 in inclusions and cytosol during aggregation and disaggregation. (A) Pseudo-color fluorescence lifetime images of cells expressing both SOD1-G85R-mTFP1 and SOD1-G85R-cp173mVenus. White arrows indicate inclusions. Cells were treated for 16 h with no reagents (a), DMSO (b), or MG-132 (c). After MG-132 treatment for 16 h, cells were transferred to the recovery culture and incubated for 10 h (d and e). Images of cells without (d) or with (e) inclusion structures are shown. (B, C) Comparison of the fluorescence lifetime of the FRET donor SOD1-G85R-mTFP1 after MG-132 treatment for 8 h (B, lanes 2–5) or 16 h (B, lanes 6–8), or in recovery culture for 10 h (C, lanes 1–4). Fluorescence lifetime values of individual cells are shown as open circles (n = 10–20), and average values are shown with bars. Significant differences were determined using Student’s t-test: ★P < 0.01, ★★P < 0.05.

    Journal: Genes to cells : devoted to molecular & cellular mechanisms

    Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1

    doi: 10.1111/gtc.12125

    Figure Lengend Snippet: FRET-FLIM analysis of mutant SOD1 in inclusions and cytosol during aggregation and disaggregation. (A) Pseudo-color fluorescence lifetime images of cells expressing both SOD1-G85R-mTFP1 and SOD1-G85R-cp173mVenus. White arrows indicate inclusions. Cells were treated for 16 h with no reagents (a), DMSO (b), or MG-132 (c). After MG-132 treatment for 16 h, cells were transferred to the recovery culture and incubated for 10 h (d and e). Images of cells without (d) or with (e) inclusion structures are shown. (B, C) Comparison of the fluorescence lifetime of the FRET donor SOD1-G85R-mTFP1 after MG-132 treatment for 8 h (B, lanes 2–5) or 16 h (B, lanes 6–8), or in recovery culture for 10 h (C, lanes 1–4). Fluorescence lifetime values of individual cells are shown as open circles (n = 10–20), and average values are shown with bars. Significant differences were determined using Student’s t-test: ★P < 0.01, ★★P < 0.05.

    Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

    Techniques: Mutagenesis, Fluorescence, Expressing, Incubation, Comparison